The Madhani Lab is in the Department of Biochemistry and Biophysics at UCSF
ucsfmadhani lab

Cryptococcus neoformans KN99α strain ​gene deletion feedback form

In order to maximize the quality of the gene knockout collection, the Madhani lab is soliciting feedback on gene deletions found to be problematic. Although each knockout strain has subject to PCR-based verifications to verify junctions and loss of coding sequence, any method has a false-positive rate. Problematic strains will be flagged and, if feasible, reconstructed.

Use the form below to submit information germane to this issue. Include the gene deletion name, plate number and well coordinates. Issues related to strain amplification (viability or contamination) may be directed to the Fungal Genetic Stock Center (

Thank you for your support.

--The Madhani lab